Fig. 3

Generation of Gpr62 knockout mice. A Schematic of conventional targeting strategy used to generate a Gpr62 knockout mouse line. Gpr62 is encoded by a single exon gene with its 3′ UTR overlapping the 3′ UTR of Pcbp4 on the opposite strand. A targeting vector was designed to insert loxP sites upstream and within the Gpr62 3′ UTR. Correctly targeted mice were crossed to FlpER and germline expressing Cre lines to generate the loxP flanked and knockout mouse lines, respectively. B PCR confirmation of generation of heterozygous and knockout mice, using the primers in (A). C qPCR expression of Gpr62 and Gpr61 expression in the brains of 8-week old control and knockouts (n = 6). Gpr62 null mice showed the expected loss of Gpr62 mRNA expression without any detectable compensatory upregulation of Gpr61. D. Gpr62 expression can be seen in chains of glial cells (presumptive oligodendrocytes) in the white matter of the spinal cord and the optic nerve of the wild-type littermates, with this expression absent in the knockout (inserts, D)