Fig. 9

Full retinal Brn3a KO significantly affects distribution of GFRα Ret co-receptors in RGCs. Retinal sections from Rax:Cre; Brn3a^CKOAP/WT (a,c,e) and Rax:Cre; Brn3a^CKOAP/KO (b, d, f) mice, were stained using antibodies against GFRα1 (a-b),αGFRα2 (c-d) or αGFRα3 (e–f) (green, left panels) in conjunction with AP (red, middle panels) and DAPI (right panels show three-color merged image). Arrowheads indicate single labelled cells (AP or GFRα) and arrows point to double labeled cells (GFRα ⁺AP⁺). Total numbers of quantified cells are represented as pie charts in g, i and k and indicated next to each sector. Significance levels for the comparison between the distributions for Rax:Cre; Brn3a^CKOAP/WT (left) and Rax:Cre; Brn3a^CKOAP/KO mice are calculated using the Chi-square statistic. Chi statistic, p-values and degrees of freedom are indicated in Supplementary Table 2. Box-plots representing proportions of different cell categories according to the expression of each GFRα and AP are shown in h, j an l, expressed as percent total DAPI positive cells in the GCL. Significance levels for differences between Rax:Cre; Brn3a^CKOAP/WT (left) and Rax:Cre; Brn3a^CKOAP/KO (right) retinas were calculated using Kolmogorov-Smirnoff 2 test. For each genotype and antibody, sections from 2–3 different animals were stained and 8–10 images were quantified (Supplementary Table 2). For Box-Whisker plots, the red lines represent the median, the rectangles represent the interquartile interval, and the whiskers the full range of observations. Outliers are indicated by red crosses. Scale bar in (f) is 25 μm