Fig. 7

Effects of Ret and Brn3a gene dosage on RGC type distribution. a-d represent summaries of data presented in Figs. 2, 3, 4, 5 and 6. RGC type is indicated on the y axis. Frequency (% total cells within genotype and age) is indicated on the x axis. Monostratified and bistratified cell types are separated by the green stippled line. Cell types are arranged top to bottom according to the proximity of dendritic arbor to the GCL. RGC type definitions and relative frequencies are indicated in Tables 1 and 2. Cell type distributions for each retina are indicated in Supplementary Table 3. a Relative frequency of observed RGC types in retinas from Ret^CreERt2/WT; ROSA26^AP/WT; Brn3a^KO/WT (gray, n = 169 cells from 6 mice, derived from 3 distinct experiments/litters) and Ret^CreERt2/WT; ROSA26^AP/WT; Brn3a^WT/WT (white, n = 138 cells from 3 mice, derived from 2 distinct experiments/litters) genotypes, as described in Fig. 6. The relative distributions of cell types in complete Brn3a^KO/WT (gray bars) vs. Brn3a^WT/WT (white bars) retinas were not statistically different from each other (χ² statistics, p = 0.328, see Supplementary Table 1). b-d RGC type frequency in retinas from Ret^CreERt2/WT; Brn3a^CKOAP/WT (blue) and Ret^CreERt2/WT; Brn3a^CKOAP/KO (red) mice in which recombination was induced at E15 (b, n = 324 and 232 cells from 10 and 8 mice, derived from 5 distinct experiments/litters), P0 (c, n = 89 and 93 cells from 3 and 2 mice) or P22 (d, n = 148 and 145 cells, from 3 and 3 mice), as described in Figs. 2, 3, 4 and 5. The relative distribution differences of cell types in sparse Brn3a^KO/WT (blue bars) vs. Brn3a^KO/KO (red bars) RGCs did not reach statistically significance at P22 (p = 0.071), but were highly significant at P0 (p = 3.9*10^–5) and E15 (p = 6*10^–7, χ² statistics comparing the distribution of RGC types for each genotype, see Supplementary Table 1). Cell type distributions for sparsely recombined Brn3a^KO/WT RGCs (blue bars) were not significantly different between P22 and P0 (p = 0.231), but highly significant between P22 and E15 (p = 1.6*10^–30, χ² statistics)