Fig. 6

Morphological characterization of RGCs from Ret^CreERt2/WT; Brn3a^KO/WT; ROSA26^AP/WT and Ret^CreERt2/WT; Brn3a^WT/WT; ROSA26^AP/WT retinas. Cre was activated by i.p. administration of 250 μg 4-HT to mothers of the pups at gestational day 15 (E15 for pups), and RGCs visualized by recombination of the ROSA26^AP reporter. Labelled RGCs have the same Ret and Brn3a gene copy numbers as their respective retinas (either Ret^KO/WT; Brn3a^KO/WT—left panels, or Ret^KO/WT; Brn3a^WT/WT—right panels). RGCs were labelled in retinas from Ret^CreERt2/WT; ROSA26^AP/WT; Brn3a^KO/WT (gray, n = 169 cells from 6 mice, derived from 3 distinct experiments/litters) and Ret^CreERt2/WT; ROSA26^AP/WT; Brn3a^WT/WT (white, n = 138 cells from 3 mice, derived from 2 distinct experiments/litters) mice. a Retinal flat mounts from Ret^CreERt2/WT; Brn3a^KO/WT; ROSA26^AP/WT (left) and Ret^CreERt2/WT; Brn3a^WT/WT; ROSA26^AP/WT (right) mice, processed for AP histochemistry. Scale bar 500 μm. b-e Scatter plots for parameters of monostratified RGCs, defined and presented as in Figs. 2 and 3. f-j Scatter plots for parameters of bistratified RGCs as defined in Fig. 5. Example cells from Figs. 2 and 4 are indicated by arrowheads and letters corresponding to each cell in Fig. 2 – for monostratified RGCs, and in Fig. 4 – for bistratified RGCs