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Fig. 3 | Neural Development

Fig. 3

From: Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification

Fig. 3

Monostratified RetCreERt2/WT; Brn3aAP/WT and RetCreERt2/WT; Brn3aAP/KO RGCs show significant developmental shifts in cell type distribution. a-h Scatter plots of morphological parameters for monostratified RGCs. RetCreERt2/WT; Brn3aCKOAP/WT (left) and RetCreERt2/WT; Brn3aCKOAP/KO (right) mice, were injected with 4-HT, as described in Fig. 1 and retinas stained and individually stained cells imaged as described in Fig. 2. RGCs were quantitated from RetCreERt2/WT; Brn3aCKOAP/WT and RetCreERt2/WT; Brn3aCKOAP/KO mice in which recombination was induced at E15 (10 and 8 mice, derived from 5 distinct experiments/litters), P0 (3 and 2 mice) or P22 (3 and 3 mice). After recombination, the labelled and measured cells become either RetKO/WT; Brn3aKO/WT (RetCreERt2/WT; Brn3aAP/WT), a-d or RetKO/WT; Brn3aKO/KO (RetCreERt2/WT; Brn3aAP/KO) e–h Measured parameters are described in Fig. 2i. a,e Normalized ID (ID/IPL, x) versus OD (OD/IPL, y). b,f Normalized ID (ID/IPL, x) versus area (y). c, g Normalized dendritic arbor thickness ((OD – ID)/IPL, x axis) versus arbor area (y). d, h Distance from optic disc to the cell body (x axis) versus area (y). The two vertical bars in (a-b) represent the theoretical lamination levels of ON and OFF starburst ACs, and the grey box represents the OFF sublamina of the IPL, where mGluR6GFP positive axons are absent (Morgan et. al, 2006). The putative morphological types are assigned based on previous work and correspondence to literature, color coded in the scatter plots, and indicated at the bottom. Example cells from Fig. 2 are indicated by arrowheads and corresponding letters

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