Fig. 10

Dynamic co-expression and cross-regulation of Ret and Brn3a during embryonic and postnatal development. a,b Immunostaining of retina sections from E15 (a) and P0 (b) Rax:Cre; Brn3a^CKOAP/WT (top row) and Rax:Cre; Brn3a^CKOAP/KO (bottom row) mice, using anti-Ret (white), anti- GFRα1 (green) and anti-alkaline phosphatase (anti-AP, red) antibodies, and DAPI nuclear stain. (c,d) Pie-charts and (e,f) box-plots representing proportions of different cell categories according to the expression of Ret, GFRα1 and AP in the GCL of retinas from Rax:Cre; Brn3a^CKOAP/WT (left) and Rax:Cre; Brn3a^CKOAP/KO (right) animals at E15 (c,e) and P0 (d,f). Total number of cells for each category are presented next to the pie-chart markers, and data spread for each category is shown in the boxplots, expressed as percent total DAPI positive cells in the GCL. (g,h) Immunostaining of retina sections from E15 (g) and P0 (h) Rax:Cre; Ret^CKCFP/WT (top row) and Rax:Cre; Ret^CKCFP/CKCFP (bottom row) mice, using anti-GFP (staining CFP expressing cells, green) together with anti-Brn3a (red), anti-Brn3b (white) antibodies, and DAPI nuclear stain. i,j Pie-charts and (k,l) box-plots representing proportions of different cell categories according to the expression of Ret, Brn3a and Brn3b in the GCL of retinas from Rax:Cre; Ret^CKCFP/WT (left) and Rax:Cre; Ret^CKCFP/CKCFP (right) animals at E15 (i,k) and P0 (j,l). For each genotype and age, sections from at least 3–5 different animals were stained and 6–13 images were quantified (Supplementary Table 2). Scale bar (h) is 25 μm. Total number of cells, medians and statistics for pie charts and box plots are indicated in Supplementary Table 2