Fig. 6

UNC-34 EVH1 domain is required for filopodia initiation and dendrite branch formation. A Representative images of PVD dendrite morphology in wild-type, unc-34 null, UNC-34 wild-type rescue, and UNC-34ΔEVH rescue animals at the L4 stage. Yellow rectangles indicate locations of the magnified views shown on the right. B Quantification of the number of quaternary branches in a region 100 µm anterior to the PVD cell body. Statistical comparison was performed using Brown-Forsythe ANOVA with Dunnett’s multiple comparisons test (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05) (n = 12, 16, 14, and 12 worms, respectively, in the order of presented genotypes). C Live animal imaging of developing PVD dendrites in L3 stage animals expressing a morphology marker. Cyan arrows indicate filopodia emerging from swelling in wild-type rescue. Yellow asterisks indicate swellings, including persistent tip swellings in UNC-34ΔEVH rescue animals, reminiscent of unc-34 null worms. Scale bar is 5 µm. D and E Quantification of filopodia and swelling formation as described in Fig. 2. Statistical comparison was performed with a Brown-Forsythe ANOVA with Dunnett’s multiple comparisons test (**, p < 0.01; *, p < 0.05; ns, p > 0.05; n = 11, 9, 19, and 11 worms, respectively, in the order of presented genotypes)