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Fig. 3 | Neural Development

Fig. 3

From: A two-step actin polymerization mechanism drives dendrite branching

Fig. 3

WRC mutants lack both filopodia and swellings. A Representative images of PVD dendrite morphology in wild-type, gex-3, gex-2, and wve-1 null animals at the L4 stage. Yellow rectangles indicate locations of magnified views on the right. B Quantification of number of quaternary branches in a region 100 µm anterior to the PVD cell body. Statistical comparison was performed using Brown-Forsythe ANOVA with Dunnett’s multiple comparisons test (****, p < 0.0001; n = 13 worms for wild-type; n = 14 worms for gex-3; n = 8 worms for gex-2, and n = 19 worms for wve-1). C Live animal imaging of developing PVD dendrites in L3 stage animals in wild-type and gex-3 animals. Yellow asterisks indicate swelling events that gave rise to filopodial growths (cyan arrows), which are largely absent in gex-3 worms. Note the overall lack of growth in gex-3 mutants compared to wild type. Scale bar is 5 µm. D Representative traces showing growth of individual dendrites of indicated genotypes. E and F Quantification of filopodia and swelling formation as described in Fig. 2. Statistical comparison was performed using two-tailed Mann–Whitney test (****, p < 0.0001; n = 10 worms for wild-type; n = 11 worms for gex-3). G and H Quantification of positive growth events in indicated genotypes as described in Fig. 2. Statistical comparison was performed using two-tailed Mann–Whitney test (**, p < 0.01; ***, p < 0.001; n = 24 branches for wild-type; n = 18 branches for gex-3)

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