Fig. 8
From: Neuronal Dystroglycan regulates postnatal development of CCK/cannabinoid receptor-1 interneurons
Constitutive deletion of Bax does not rescue CB1R+ terminals in the hippocampus. (a) Breeding scheme for deletion of Bax in Dag1Control and Dag1cKO mice; the four genotypes analyzed and their abbreviations are shown to the right. (b-e) Coronal sections of the hippocampus stained for CB1R (gray) from (b) Dag1Control;BaxControl, (c) Dag1Control;BaxKO, (d) Dag1cKO;BaxControl and (e) Dag1cKO;BaxKO mice. (B′-E’) Enlarged images of the CA1 (yellow boxed regions) stained for CB1R (green; Right, gray single channel images) and Hoechst (magenta). (f) Quantification of CB1R pixels in hippocampal CA1 layers from Dag1Control;BaxControl (black bars), Dag1Control;BaxKO (gray bars), Dag1cKO;BaxControl (purple bars), and Dag1cKO;BaxKO (pink bars) shows that deleting Bax fails to rescue the loss of CB1R in Dag1cKO mice (n.s. = not significant; *P < 0.05, unpaired two-tailed Student’s t-test; n = 2–5 mice/genotype). Data are presented as mean values ± s.e.m. Data are normalized to Dag1Control;BaxControl signal in each CA1 layer. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum