Fig. 7

Ablation of NSPCs blocks spinal cord regeneration. a Diagram of the treatment of zGFAP::mCherry-NTR transgenic animals with metronidazol (MTZ) or vehicle, followed by spinal cord resection, swimming recording, and histological analysis. b-e Eye imaging (b, d) before treatment, and 7 days after incubation with c vehicle or e MTZ. f, g Spinal cord sections showing mCherry expression f before, and g 7 days after MTZ treatment. h Graph of swimming at 1, 10, 15, 25 dpr in sham (Sh) operated animals treated with MTZ (Sh-MTZ, blue boxes), and resected (Rs) animals incubated with vehicle (Rs-Vehicle, red boxes) or MTZ (Rs-MTZ, green boxes). i-l Immunofluorescence against Sox2 (green) and nuclei stained with Hoechst of spinal cord sections obtained from animals at 30 dpr from the i, j Rs-vehicle, and k, l Rs-MTZ treated groups. Statistics in graph H: ANOVA-one way with Bonferroni post-test, ** p < 0.01, n = 4 independent biological replicates