Fig. 5
Response to injury of Neural Stem and Progenitor Cells. a Scheme of EdU treatment. b Click-iT staining for EdU (red), and immunofluorescence against EGFP (green), merge with nuclei (blue) in sham control animals at 2 days post sham operation (dps), and 2 dpt. c Graph of EdU-EGFP positive cells per mm3 at 2 dps (red bar) and 2 dpt (green bar). t-Test, ***: p < 0.001. d, e, f, g, h, i Immunofluorescence against EGFP (green) at NF stage 50 in d uninjured, e 2 days post resection (dpr), f 6 dpr, g 7 dpr, h 10 dpr, and i 20 dpr. Magnifications are shown in panels d’-d”, e’-e”, f’-f”, g’, h’ and i’-i”. j, k, l, m, n, o. Serial sections from the same preparation shown in panels d, e, f, g, h, i double stained for EGFP (green) with the neuronal marker Acetylated tubulin (red), and merge (orange). Nuclei are label in blue with Hoechst. White arrowhead highlights colocalization. Scale bar: d, e, f, g, h, i: 200 μm; d’-d”, e’-e”, f’-f”, g’, h’, i’-i”, j-j”, k-k”, l-l”, m-m”, n-n”, o-o”: 50 μm