Fig. 4

Zebrafish regulatory regions of GFAP drive expression of EGFP in neural stem and progenitor cells, and astrocytes in Xenopus laevis spinal cord. A-C Lateral view of EGFP expression in the central nervous system at A, B NF-Stage 43, and C NF-Stage 50. A EGFP expression in the eye, brain and spinal cord (arrowheads). B EGFP/brightfield merge. C Dorsal view of EGFP expression in the optic tectum, hindbrain and spinal cord at NF stage 50. D-F Double staining against D EGFP and E Sox2. Panels F showed merge image, and F’, F” magnifications of the dorsal and ventral cells surrounding the central canal. G-O’ Characterization of EGFP cells by double staining at NF stage 66. G-I” EGFP and Sox2; J-L’ EGFP and Brain lipid-binding protein (BLBP); and M-O’ EGFP and Glutamine synthase (GS). Nuclei are label in blue with Hoechst. P-Q Immunogold staining against EGFP at NF stage 50. P EGFP⁺ cell in contact with the central canal. P’ Magnification of square in P. Expression of EGFP is visualized by the black dots of the gold staining. P” Magnification of square in P’. Gold staining (black arrowhead) in close apposition with filaments (white arrowhead). Q Endfeet from an EGFP⁺ cell (colored green) in close contact with blood vessel (colored red). R Gene ontology analysis of the RNAseq from EGFP⁺ cells revealed the stem cell/neural precursor cell identity of these cells. S Dendrogram of EGFP⁺ cells and EGFP⁻ cells showing the hierarchical clustering of EGFP⁺ cells with astrocytes and EGFP⁻ cells with neurons and oligodendrocytes. Scale bar: C, F’-F″: 20 μm; A-B, D-F, I’-I″, L’, O’: 50 μm; G-I, J-L, M-O: 200 μm