Fig. 3

Glial cell and extracellular matrix response to spinal cord injury in R-Stage and NR-Stage. a-c Immunostaining against vimentin in a uninjured, and at b 2 and c 6 dpt from animals at NF stage 50. d Western blot against Vimentin (Vim) and GAPDH of spinal cords samples obtained from uninjured (ui), and at 2, 6, 10 and 20 dpt in animals at NF stage 50. e-g Immunostaining against Vimentin in uninjured (e-e’), and at (f, f’) 10 and (g-g’) 20 dpt from animals at NF stage 66. h Western blot against Vim and GAPDH of spinal cords samples obtained from uninjured (ui), and at 2, 6, 10 and 20 dpt in animals at NF stage 66. i-o Immunofluorescence against fibronectin in i uninjured, j 6 dpt, and k 10 dpt in NF stage 50; and l uninjured, m 10 dpt, and n 30 dpt in animals at NF stage 66. o-p Immunofluorescence against CSPG in o uninjured, and p-p’ at 40 dpt in NF stage 66. q-v AFOG staining shown Collagen (blue), cells (orange) and Fibrin (red) in q uninjured, and at r 6 and s 10 dpt in animals at NF stage 50, and in t-t’ uninjured, and at u-u’ 10 and v-v’ 20 dpt from animals at NF stage 66. w Analysis of gene expression change upon spinal cord injury comparing injured animals (Ts) with control sham (sham) surgery at 1, 2 and 6 days after injury in NF stage 50 (1R, 2R and 6R), and NF stage 66 (1NR, 2 NR and 6 NR). Colored and crosses scale indicates the level of increase upon injury in green (+, ++, +++) and decrease in red (−, −−, −−−), data obtained from a previous RNAseq analysis [49]. The red dotted lines (b, f, g, j, m, n, p, r, u, v) and yellow arrows (c, k, s) indicate the injured site. Nuclei stained with Hoechst in blue (a-c; e-g; i-p)