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Fig. 5 | Neural Development

Fig. 5

From: The SLC36 transporter Pathetic is required for neural stem cell proliferation and for brain growth under nutrition restriction

Fig. 5

Effects of nutrition restriction on pathNRE and E(spl)mγ-GFP expression. (A) Scheme for experiments with nutrition restriction (NR). Larvae were transferred to amino acid-deprived diet at 72 h (ALH) and dissected at 120 h ALH. (B-E) pathNRE expression is reduced under NR conditions. pathNRE-GFP (green, grey panel) expression in VNC at 72 h ALH (B), 120 h ALH fed with yeast-rich food (C) and 120 h under NR from 72 h to 120 h ALH (D). NBs are marked with Dpn (red) and Mira (blue). (E) Quantification of pathNRE-GFP intensity in above conditions (n = 18,16,22). (F-I) Neural stem cells retain Notch activity in response to NR. (F-H) Notch regulated E(spl)mγ-GFP (green, grey panel) expression in VNC at 72 h ALH (F), 120 h ALH fed (G) and 120 h with NR from 72 h to 120 h ALH (H). NBs are marked with Dpn (red), neuronal progeny are marked with Pros (blue). (I) Quantification of EE(spl)mγ-GFP intensity in above conditions (n = 15,14,20). ****p < 0.0001; ns, not significant (p > 0.05). Scale bar, 50 μm

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