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Fig. 3 | Neural Development

Fig. 3

From: The SLC36 transporter Pathetic is required for neural stem cell proliferation and for brain growth under nutrition restriction

Fig. 3

Depletion of Path causes an increase in NSC cell size and a reduction in proliferation. (A) Scheme for mosaic clone induction. Clones were induced (heat-shock treatment) 48 h after larval hatching (ALH) and larvae dissected 72 h after clone induction (ACI). (B) Mosaic clones from control and path mutants. NBs are marked with nuclear Dpn (red) and membrane associated Mira (blue & grey). GFP-marked NB lineages are outlined with yellow dotted lines. (C) Number of progeny per labelled lineage, n = 26, 42. (D) Quantification of NB sizes, measured using Mira, from controls and path mutants; n = 253, 120. (E) EdU labelling of control and path mutant NB clones 3 days ACI. Cells undergoing DNA synthesis during 4 h of EdU incubation are labelled (red and grey panels); Nuclei are marked by Hoechst (blue) staining. GFP-marked NB lineages are outlined with yellow dotted lines. (F) Quantification of EdU-positive (EdU+) cells in marked NB lineages; n = 61, 51. (G) Quantification of GMCs (Asense +ve) in marked NB lineages. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar, 50 μm

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