Fig. 2

A Notch responsive element (NRE) in path directs expression in neural stem cells. (A) A genomic overview of the path gene region with the Su(H) binding profile from Nact (N∆ECD)-expressing brains. Graph depicts Su(H) bound regions (enrichment relative to input AvgM, scale log2 0–4) in NΔECD brains (green). Blue bars indicate Su(H) binding motifs identified using Patser, height of bar represents Patser score, scale 5–9.79 [26]. Gene models are depicted in blue and cloned pathNRE region in cyan. (B) Strategy for mutating high-affinity Su(H) binding motif in pathNRE. (C) The response of pathNRE to transient activation of Notch in S2 cells. (NRE): positive control, E(spl)m3NRE, a known Notch target enhancer; (NME): negative control, Notch mutated enhancer (NME). Error bars represent the SD of three biological replicas; *p < 0.05. (D&E) Dorsal (D) and ventral (E) expression from pathNRE-GFP reporter detected with anti-GFP in L3 larval brains. Scale bar, 100 μm. (F-J) Reporter expression from the indicated genotypes detected with anti-GFP (green and grey panel). Scale bar, 50 μm