Fig. 6

Nkx6 is necessary and sufficient to specify VO motor neuron molecular identity. (A) Controls (NB7–1-gal4 UAS-myr:GFP) have one Nkx6+ Eve− VO motor neuron. (B) Nkx6 overexpression (NB7–1-gal4 UAS-myr:GFP UAS-Nkx6) results in ectopic VO motor neurons at the expense of Eve+ U3-U5 motor neurons. (C) Nkx6 RNAi (NB7–1-gal4 UAS-myr:GFP UAS-Nkx6-RNAi) lack the VO motor neuron and possess an ectopic Eve+ “U?” motor neuron at that location (cyan arrowhead). (D, D’) Quantification. (E) Summary. Scale bars, 5 μm