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Fig. 1 | Neural Development

Fig. 1

From: The first quarter of the C-terminal domain of Abelson regulates the WAVE regulatory complex and Enabled in axon guidance

Fig. 1

Hem and Sra-1 bind the first quarter (1Q) of Abl and genetically interact with Abl. a GST-1Q pulldown from Drosophila embryonic lysate, showing spectral counts of significantly enriched proteins from 3 replicates from mass spectrometry analysis. b-gHem and Sra-1 genetically interact with Abl at the midline. b Wild-type embryo stained with mAb 1D4. The cAbl and dHem homozygotes show crossing over defects (black arrowheads) at the midline. e Heterozygosity for Abl rescues Hem homozygotes, while g heterozygosity for Sra-1 rescues Abl homozygotes. f The Hem Abl double homozygote is severely perturbed, with major defects in projections of both commissural and longitudinal axons. h Schematic diagram for motoneurons of abdominal segments A2-A7. The midline is at the bottom, and anterior is to the left. Note innervations of the ISNb (red) with muscles 7, 6, 13, and 12. i-lHem and Abl genetically interact in motoneuron axon guidance. (I) Wild-type ISNb projection in abdominal hemisegments A2–7. jAbl and kHem homozygotes occasionally have stop short defects where the ISNb fails to innervate muscles 12/13 (black arrows). lHem Abl homozygotes have a severe increase in stop short defects and other axon guidance defects (white arrows). Pictures are representative of 3 sets of embryo collections

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