Fig. 7

Co-localization of bHLH-induced activity of ECR9 and ECR65 with early retinal development markers. E5 retinas were electroporated with ECR9::GFP or ECR65::GFP and their corresponding bHLH factors and cultured for 18–22 h before harvest and immunohistochemistry. (a) Cell quantitation derived from confocal images of retinas stained for co-electroporation marker Bgal, and either Vsx2, Otx2 or OC1. Percentages of Otx2(+), Vsx2(+), or OC1(+) cells were calculated out of the total number of Bgal(+) cells. (b) Cell quantitation derived from confocal images of retinas stained for ECR9::GFP, co-electroporation marker Bgal, and either Vsx2, Otx2 or OC1. Percentages of ECR9(+) Otx2(+), ECR9(+) Vsx2(+), or ECR9(+) OC1(+) cells were calculated out of the total number of Bgal(+) cells. Error bars represent 95% CI (c) Confocal images of retinas stained for ECR9::GFP and Otx2. Yellow arrows denote ECR9(+) Otx2(+) cells. White arrows denote ECR9(+) Otx2(−) cells. (d, e) Same as for (a, b) but for ECR65::GFP. f Confocal images of retinas were stained for ECR65::GFP and Vsx2. Yellow arrows depict ECR65(+) Vsx2(+) cells. White arrows depict ECR65(+) Vsx2(−) cells. Scale bar in (c,f) represents 50 μm and applies to all panels. Scale bar in insets represents 25 um and applies to all panels