Fig. 6
From: Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells
Onset of ECR9 and ECR65 activity compared to ThrbCRM1 (a) E5 retinae were electroporated with ThrbCRM1::TdT and either ThrbCRM1::GFP, ECR65::GFP or ECR9::GFP and cultured for 8 h before harvest. Immunohistochemistry was used to amplify GFP and TdT signal. (b) Quantification of ThrbCRM1(+) cells within ECR9(+) population and ECR65(+) population. Each data point represents a biological replicate. Error bars represent 95% confidence interval (c) E5 retinae were electroporated as in (a) but also stained to detect Vsx2. Yellow arrow indicates a cell that is ThrbCRM1::TdT(−), ECR9::GFP(+), Vsx2(+). (d) E5 retinae electroporated with ECR9::GFP and ThrbCRM1::TdT were cultured for 8 h and pulsed with EdU from hour 7–8. Yellow arrow indicates a cell that is ECR9::GFP(+) ThrbCRM1::TdT(−) EdU(+). White arrow indicates a cell that is ECR9::GFP(+) ThrbCRM1::TdT(−) EdU(−). Scale bars in (a, c, d) represent 50 μm and apply to all panels. Scale bars in (c) and (d) insets represent 20 μm and apply to all panels
