Fig. 5
From: Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells
Effect of overexpression of bHLH factors on regulatory activity of ECR9, ECR65, and ThrbCRM1. (a) E5 retinae were electroporated with Nhlh1, NeuroD1, NeuroD4, NeuroG2 or Atoh7 under the control of the CAG promoter in combination with ECR65, ECR9 or ThrbCRM1 driving either TdT or GFP. Percentages of ECR9(+), ECR65(+) and ThrbCRM1(+) cells were calculated out of the total cells marked by co-electroporation control CAG::IRFP. (b) Retinae were electroporated with ECR9::GFP or ECR65::GFP in combination with ThrbCRM1::AU1 and a CAG::bHLH plasmid. (c, d) Comparison of the effect of bHLH overexpression on mutant and WT versions of ECR9 and ECR65. Percentages were calculated out of total number of CAG::IRFP(+) cells. Percentages of WT enhancer(+) cells in the control condition were scaled to 100% for (c, d). Percent of enhancer(+) cells in the empty CAG vector control condition were normalized to 100% for a. Error bars represent 95% confidence interval. In all graphs, each datapoint represents a biological replicate. See Methods for statistical tests