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Fig. 6 | Neural Development

Fig. 6

From: Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function

Fig. 6

Ena is required to regulate clustering of synaptic vesicles, but not average vesicle size at the T-bar. a-g, Analysis of electron micrographs of type 1b synaptic boutons at the 6/7 NMJ. Representative image of T-bar AP4mito control (a) and FP4mito (enaLOF, b) under control of the neuronal 1407-GAL4 driver. The dashed line (a,b) indicates 200 nm radius from the center of the T-bar. A significant increase in average SV number is observed in FP4mito animals within this region (enaLOF, c). d-f, Abnormally-shaped and enlarged SVs within 200 nm from the electron dense adhesive contact of the active zone (indicated by white brackets) were observed in FP4mito (enaLOF, white arrow heads, e-f) in contrast to controls (d). Although average area of synaptic vesicles is unchanged in FP4mito (enaLOF) animals, the distribution of SV area (g) indicates rare large vesicles in these animals (orange distribution), which are not observed in control (blue distribution). * P < 0.05, as determined by Welch’s t-test; error bars indicate ± s.e.m. of genotype; gray shading indicates ± s.e.m. of control; n = 3 animals for all genotypes; scale = 100 nm

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