Fig. 4
From: Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function
Presynaptic Ena regulates active zone structure. Electron micrographs of type 1b synaptic boutons at the 6/7 NMJ from flies expressing UAS-AP4mito (control, a) and UAS-FP4mito (enaLOF, b) under the control of the neuronal 1407-GAL4 driver were obtained to analyze gross, qualitative ultrastructure (a,b) and to quantify active zone area (c,d). Qualitative comparison revealed no catastrophic differences in SSR (pink shading) or bouton (yellow shading) morphology and/or size in enaLOF (b) compared to controls (a). To determine quantitative phenotypes, mean active zone area was calculated by adding length of the electron dense region multiplied by the thickness of the serial sections (100 nm) for all sections spanning the active zone (c). D, Mean active zone area is significantly increased in enaLOF. M indicates mitochondria; ** P < 0.01, as determined by Welch’s t-test; error bars indicate ± s.e.m. of genotype; gray shading indicates ± s.e.m. of control; n = 3 animals for all genotypes; scale bar = 500 nm