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Fig. 1 | Neural Development

Fig. 1

From: Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function

Fig. 1

Presynaptic Ena expression is required to promote neuromuscular junction development. Fluorescence images (a-b) and quantification (c-d) of NMJs from muscle 6/7 in segment A2 of third-instar wandering larvae. Flies expressing UAS-AP4mito (control; A-A") and UAS-FP4mito (enaLOF; B-B") under the control of the neuronal 1407-GAL4 driver are shown stained with horseradish peroxidase (HRP; green, top panels), Futsch (red, middle panels), and with the HRP/Futsch channels merged (yellow, bottom panels). C, Quantification of synaptic 1b and 1 s bouton number in neuronal enaLOF lines demonstrate a statistically significant decrease relative to control. Expression of UAS-Ena(+) under the control of 1407-GAL4 rescues the loss of bouton number in enaLOF animals (c). D, Branch number is also significantly decreased enaLOF. * P < 0.05, as determined by Welch’s t-test; error bars indicate ± s.e.m. of genotype; gray shading indicates ± s.e.m. of control; n ≥ 20 NMJs for all genotypes, scale = 20 μm

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