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Fig. 4 | Neural Development

Fig. 4

From: An inducible Cre mouse line to sparsely target nervous system cells, including Remak Schwann cells

Fig. 4

ECSF mice sparsely label astrocytes and neurons but not microglia and oligodendrocytes in the brain. a-d Brains from tamoxifen- and vehicle-injected ECSF; Ai14 mice were immunostained for astrocytes (Gfap), neurons (NeuN), microglia (Iba1) and oligodendrocytes (CNPase), and nuclei (DAPI, blue). Lower rows are enlarged views of the boxed regions showing representative targeted (arrow) and untargeted (arrowheads) cells in a and b, and representative untargeted cells (arrowheads) in C and D. Scales bars = 50 μm. e Cre activity is absent in the brain of vehicle-injected mice. Scales bar = 50 μm. f Quantification of cells in A-D shows that 6.8% of Gfap-positive, 11.1% of NeuN-positive, 0% of Iba1-positive and 0% of CNPase-positive cells are targeted in the cortex of tamoxifen-receiving mice. Three mice; 4 sections/mouse; error bars, standard errors of mean

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