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Fig. 6 | Neural Development

Fig. 6

From: Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life

Fig. 6

Characteristics of the Lrig1+ neurogenic stem cells. a The cells are shown in depth pseudocolor maximum projection to reveal their location in the lateral wall. E, ependymal cell. Scale bar, 10 μm. b Ventricular surface as revealed by the β-catenin and γ-tubulin staining. Scale bar, 10 μm. c The different types of RFP+ cells are shown at different depths of the lateral wall. Scale bar, 10 μm. d Histogram of distance between cell center and ventricular surface measured from orthogonal views of high resolution confocal z-stacks (see orthogonal view in c). e Dot plot of the distance between cell center and ventricular surface. Mean ± standard deviation. f-g” Lrig1+ cell lineages and Pdgfra. f-f” RFP and Pdgfra at the ventricular surface. Scale bar, 10 μm. g-g” RFP and Pdgfra deep in the lateral wall. Scale bar, 10 μm. h-k” Lrig1+ cell lineages and Gfap. h, i, j, k The cells are pseudo-colored based on the distance from the ventricular surface to highlight their position in the lateral wall. h-h” Gfap+ α/β subtype cells. Scale bar, 10 μm. i-i” Gfap+ tanycytes. Scale bar, 10 μm. j-j” Gfap- α/β subtype cells. Scale bar, 10 μm. k-k” Gfap- tanycytes. Scale bar, 10 μm. l Percentage of Gfap protein in α/β subtype cells and tanycytes. mLrig1 and Gfap in single cell RNA sequencing clusters. n Mean of expression levels in cells of each subcluster. o Graph of the mean expression levels

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