Fig. 4

Identification of the Lrig1+ neurogenic stem cells. a-b’ Potential stem cells labeled by the driver. a-a’ α morphotype cell. Note the absence of Ki-67 and EdU signal after 1 week of EdU administration in drinking water. Note the branches and the long basal process (arrows). Note the RFP+ neuroblast (NB) nearby. Scale bar, 10 μm. b-b’ β morphotype cell. Ki-67- and EdU-. Note the branches and short basal process. Scale bar, 10 μm. c’-g’ Non-stem cells labeled by the driver. c-c’ A tanycyte at the ventricular surface. Scale bar, 10 μm. d-d’ A niche astrocyte deep in the lateral wall. Scale bar, 10 μm. e-e’ An ependymal cell at the lateral surface. Scale bar, 10 μm. f-f’ A striatal neuron deep in the lateral wall. Scale bar, 10 μm. g-g’ A mural cell – a pericyte or a smooth muscle cell. Scale bar, 10 μm. h-i RFP+ EdU+ label retaining cells after 1 week of EdU administration and 2 weeks of chase. A Gfap+ RFP+ EdU+ cell. h and a Sox2+ RFP+ EdU+ cell. i Orthogonal views and zoomed views are below. Scale bar, 10 μm. j An RFP+ EdU+ Ascl1+ Ki-67+ singlet cell is shown (arrow). Note the large nucleus compared to the nearby cells. Orthogonal views and zoomed views are below. Scale bar, 10 μm. k Clear morphologies demonstrated in cells labeled sparsely and imaged at high resolution. Scale bar, 10 μm. l A representative whole mount showing the robust labeling of the α/β subtype cells throughout the lateral wall. m Labeling density of α/β subtype cells in the lateral wall. Mean ± standard deviation