Fig. 3
An Lrig1+ cell lineage remains neurogenic throughout adult life. a Lrig1T2A-iCreERT2/+; ROSA26Ai14/+ mice induced with a single injection of 230 mg/kg tamoxifen (TMX). Lateral walls visualized at day 3 by whole mount immunofluorescence and confocal microscopy. Scale bars, 100 μm. b Reproducible induction efficiency. Mostly non-proliferative cells were labeled. Ki-67+ RFP+ cells, Mcm2+ RFP+ cells, and Ascl1+ RFP+ cells were very rare. Each n is one lateral wall from one mouse. Mean ± standard deviation. c An RFP+ NeuN+ granule cell in the olfactory bulb. Scale bar, 10 μm. d An RFP+ NeuN+ periglomerular cell in the olfactory bulb. Scale bar, 10 μm. e-e’ Zoomed out image of the olfactory bulb showing numerous RFP+ NeuN+ cells with the morphology of interneurons. Scale bar, 100 μm. f Counts of RFP+ NeuN+ interneurons from serial sections of an olfactory bulb from a mouse demonstrating that more than one hundred RFP+ interneurons were labeled per 50 μm section of the olfactory bulb. Mean ± standard deviation. g Dcx+ pixel area in the lateral wall over time. Mean ± standard deviation. h Dcx+ RFP+ cells density (cells/mm2) in the lateral wall over time. Mean ± standard deviation. i Numbers of RFP+ cells in proliferating clusters over time. Mean ± standard deviation. Inset, total number of clusters per mouse. Mean ± standard deviation. j-j” An experimental mouse at 1 year after tamoxifen induction. j Dcx immunoreactivity. Scale bars, 100 μm. j’ Dcx-co-localized RFP immunoreactivity. Scale bars, 100 μm. j” Extent of the RFP+ Dcx immunoreactivity, i.e., readout of the neurogenesis from the Lrig1+ cell lineage. Scale bars, 100 μm