Fig. 12

ipRGC subtypes are born at the same rate and frequency. a Top, GFP (green) and LacZ (cyan) immunohistochemistry in Adult Opn4^LacZ/+; Opn4-GFP retinas labeled for EdU (magenta). Yellow arrowheads point to EdU positive M1 cells (GFP+, LacZ+) and white arrows to EdU-positive M2 cells (GFP+, LacZ-). Bottom, Schematic of ipRGC subtypes labeled with each marker in experiment. b GFP (green) and SMI-32 (red) immunohistochemistry in Adult Opn4^Cre/+;ZEG retinas labeled for EdU (magenta). Blue arrowheads indicate EdU-positive M4 cells (GFP+, SMI-32+), yellow arrows indicate EdU positive non-M4 ipRGCs (GFP+, SMI-32-), and grey arrowheads indicate EdU positive OFF-alpha RGCs (GFP-, SMI-32+). Bottom, Schematic of ipRGC subtypes labeled with each marker in experiment. c Accumulation plot of proportion of ipRGC subtypes that are EdU positive when exposed to EdU at different embryonic timepoints. Accumulation was calculated based on adding together the proportion data calculated for each timepoint. d Proportion of ipRGC subtypes that are EdU positive when exposed to EdU at specific embryonic timepoints. e Accumulation plot of proportion of M4 ipRGCs, non-M4 ipRGCs, and OFF alpha RGCs that are EdU positive when exposed to EdU at different embryonic timepoints. Accumulation was calculated based on adding together the proportion data calculated for each timepoint. f Proportion of M4 ipRGCs, non-M4 ipRGCs, and OFF alpha RGCs that are EdU positive when exposed to EdU at specific embryonic timepoints. Graphs are Mean ± SD, n = 3–4 retinas per timepoint. Scale bar is 100 μm