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Fig. 1 | Neural Development

Fig. 1

From: Cellular properties of intrinsically photosensitive retinal ganglion cells during postnatal development

Fig. 1

M1, M2, and M4 subtypes can be identified using immunohistochemistry and dendritic stratification at P6. a M1 ipRGCs filled with neurobiotin (top panel) at P6 (left) and Adult (right) stages. Cells were immunolabeled for SMI-32 (blue, right panel). M1 ipRGCs are SMI-32 negative. Bottom panel: Orthogonal view of dendritic stratification from filled M1 cell (green; top panel) at P6 (left) and Adult (right). ChAT-positive amacrine cells are shown in magenta. b Dendritic depth measurements for M1 ipRGCs at P6 (yellow), P8 (light gray), P10 (dark gray), P14 (orange), and Adult (red) ages (n = 3 cells/age). c M2 ipRGCs filled with neurobiotin (top panel) at P6 (left) and Adult (right) stages. Cells were immunolabeled for SMI-32 (blue, right panel). M2 ipRGCs are SMI-32 negative. Bottom panel: Orthogonal view of dendritic stratification from filled M2 cell (green; top panel) at P6 (left) and Adult (right). ChAT-positive amacrine cells are shown in magenta. d Dendritic depth measurements for M2 ipRGCs at P6 (yellow), P8 (light gray), P10 (dark gray), P14 (orange), and Adult (red) ages (n = 3 cells/age). e M4 ipRGCs filled with neurobiotin (top panel) at P6 (left) and Adult (right) stages. Cells were immunolabeled for SMI-32 (blue, right panel). M4 ipRGCs are SMI-32 positive. Bottom panel: Orthogonal view of dendritic stratification from filled M4 cell (green; top panel) at P6 (left) and Adult (right). ChAT-positive amacrine cells are shown in magenta. f Dendritic depth measurements for M4 ipRGCs at P6 (yellow), P8 (light gray), P10 (dark gray), P14 (orange), and Adult (red) ages (n = 3 cells/age). White arrows point to soma. Light gray shading indicates OFF sublamina. ChAT: Choline Acetyltransferase. Ganglion cell layer (GCL) and inner nuclear layer (INL) refer to middle of respective cell body layer. Scale bar is 50 μm

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