Fig. 9
Zonal patterning of neurons and glia is delayed in developing Car8wdl mice. (a) Schematic of HSP25 expression in a whole mount and a superficial coronally sectioned P17 posterior cerebellum. The dotted horizontal line transecting the whole mount schematic represents the location at which the cerebellum was cut to get the coronal sections below. (b) Modified Golgi-Cox staining in control mice showing that Purkinje cells and Bergmann glia are located together in the Purkinje cell layer (n = 5). Bergmann glia have long processes that facilitate granule cell migration and Purkinje cell dendritic elaboration. The scale bars represent 200 μm (Purkinje cell and Bergmann glia, top left), 100 μm (enlarged Purkinje cell and Bergmann glia (bottom left); Bergmann glia (middle)), and 50 μm (enlarged Bergmann glia, right). The Bergmann glia are labeled as BG. (c) Purkinje cell somas (yellow arrowheads) are abnormally aligned in Car8wdl cerebella (n = 4), but not in control cerebella (n = 3) at P17. The scale bars represent 500 μm. (d) Double immunostaining in Car8wdl; NpyGFP transgenic mice with HSP25 and GFP reveal a zebrin II-like patterning in control (n = 3) and Car8wdl cerebella (n = 4). The scale bar represents 100 μm