Fig. 4
EGL proliferation in the Car8wdl mutants recovers by P20. (a) Schematic of tissue orientation and quantification location. White and yellow dotted lines delineate inner EGL boundaries of lobules V and VI. The scale bar represents 50 μm. (b) Granule cell proliferation is abnormal in developing Car8wdl mice. * p < 0.05; ** p < 0.01; **** p < 0.0001; One-way ANOVA; Tukey’s multiple comparisons post-hoc test; Mean ± SEM. (c-d) There are significantly fewer proliferating cells total and per 50 μm in the EGL of Car8wdl mice (n = 8) than in the EGL of control mice (n = 9) at P5. * p < 0.05; Student’s t-test; Mean ± SEM. (e-f) The number (no) of proliferating cells total and per 50 μm in the EGL of Car8wdl mice (n = 8) is not significantly different from that in the EGL of control mice (n = 7) at P10. p = 0.7691; Student’s t-test; Mean ± SEM. (g-h) There are more proliferating cells total and per 50 μm in the EGL of Car8wdl mice (n = 11) than in the EGL of control mice (n = 7) at P15. * p < 0.05; Student’s t-test; Mean ± SEM. (i-j) The number (no) of proliferating cells total and per 50 μm in the EGL of Car8wdl mice (n = 7) is not significantly different from that in the EGL of control mice (n = 5) at P20. p = 0.9930; Student’s t-test; Mean ± SEM. The scale bar represents 50 μm