Purkinje cells in Car8wdl mice do not express carbonic anhydrase VIII (CAR8). (a-c) Purkinje cells in P5 control mice (n = 3) express both calbindin and CAR8. (d-f) Calbindin and CAR8 are co-expressed in Purkinje cells as seen in lobules V and VI in P5 control mice (n = 3). (g-i) Purkinje cells in Car8wdl mutant mice expressed calbindin, but not CAR8, as seen in lobules V and VI at P5 (n = 3). The scale bar represents 50 μm.