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Table 1 Characteristics of CRISPRs targeting prdm12b, bhlhe22 and nkx6.1

From: Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain

Target gene

Start Coordinate

Target sequence

Enzyme

Strand

Mutagenesis Ratea

Transmission Rateb

prdm12b

Chr5:66656496

GCTGGGGGAACACCTGTTCG

Taq1α

+

1/4

71/92 um318

43/79 um319

bhlhe22

Chr24:25069884

TTCACACACAAAGATCCGGT

BstYI

–

6/14

24/37 um320

nkx6.1

Chr21:17886500

AGTGGAGGATGCTGGTCCAG

AvaII

–

8/12

18/21 um321

  1. aThe fraction of screened F0 animals that carried a mutagenic event
  2. bThe fraction of screened F1 animals that were heterozygous for a mutagenic event
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