Fig. 3

No evidence for axon guidance defects and cleaved Caspase 3-mediated cell death in MMC neurons of Ebf2 KO mice. a Motor neuron axons (bundled into nerves) are visualized with the Hb9::GFP transgene in WT and Ebf2 KO spinal cords at e12.5 and e15.5. The MN axons that belong to MMC (dorsal nerve) are indicated with a white dotted box. Hb9::GFP signal is shown in white for better contrast. N = 4. The spinal cord boundary is indicated with a white dotted line. b Representative images of WT and Ebf2 KO spinal cords (cross-sections) immunostained with antibodies against Cleaved-Caspase 3 (C-Casp3 in green) and Lhx3 (MMC marker in red) at e12.5. The location of MMC neurons is circled. c Quantification of the number of MMC neurons (Lhx3⁺) that are also positive for C-Casp3 staining in WT and Ebf2 KO spinal cords. This analysis was performed at e12.5 and e13.5. N. S. indicates no significant differences were found with Student’s t-test. N = 4