Fig. 2

Characterization of MMC neurons in Ebf2 KO animals. a The expression of genes involved in ACh biosynthesis (VAChT, Acly), as well as the expression of the MMC-specific genes (Ldb2, Lifr) is not affected in MMC neurons (white dotted circles) of Ebf2-KO spinal cords at e13.5. N = 3. b RNA ISH analysis on Ebf2-KO spinal cords at e12.5 reveals no compensatory up-regulation of Ebf1, Ebf3 and Ebf4 transcripts in MMC neurons (white dotted circles). Similar results were obtained at e13.5. N = 3. c Two representative images are shown from e13.5 WT and Ebf2 KO spinal cords. The number of MMC neurons co-labeled with Lhx3 (red) and Hb9 (green) is not affected in Ebf2 KO spinal cords at e11.5 and e12.5, but is significantly decreased at e13.5 and e15.5. Student’s t-test was performed. *: p < 0.05, **: p < 0.01. N = 4–7. The location of MMC neurons is indicated with a dotted white circle