Fig. 10

Trajectory of cholinergic PBG axons in the WT and math5^−/− mice. Examples show a unilateral viral tracer (FLEX-AAV-ChIEF-tdT) injection into PBG of an adult WT (A-C) and math5^−/− (D-F) ChAT-Cre mice, and the corresponding axonal labeling in the supraoptic decussation (SOX) at the level of cerebral peduncle (CP) and ventral midline. a Injection of viral tracer in WT ChAT-Cre mouse resulted in robust tdT labeling of neurons (A’), restricted to the left PBG (A”). b View of the cerebral peduncles, showing PBG axons traveling in the ipsilateral supraoptic decussation (B), and running dorsally in the contralateral optic tract (B′, arrow). c View of the ventral midline showing PBG axons crossing in the supraoptic decussation and running in the optic tract. d Injection of the viral tracer in math5^−/− mouse resulted in robust tdT labeling of neurons of the left PBG. e In math5^−/− mice, PBG axons travel in the supraoptic decussation ipsilaterally (E), and run dorsally within the neuropil of the contralateral thalamus (E’, arrow). f View of the ventral midline showing PBG crossing in the supraoptic decussation and running laterally along the ventral aspect of the brain. Scale bars = 100 μm, 20 μm (A’ & D’)