- Research article
- Open Access
Dynamic Notch signalling regulates neural stem cell state progression in the Drosophila optic lobe
Neural Developmentvolume 13, Article number: 25 (2018)
Neural stem cells generate all of the neurons and glial cells in the central nervous system, both during development and in the adult to maintain homeostasis. In the Drosophila optic lobe, neuroepithelial cells progress through two transient progenitor states, PI and PII, before transforming into neuroblasts. Here we analyse the role of Notch signalling in the transition from neuroepithelial cells to neuroblasts.
We observed dynamic regulation of Notch signalling: strong activity in PI progenitors, low signalling in PII progenitors, and increased activity after neuroblast transformation. Ectopic expression of the Notch ligand Delta induced the formation of ectopic PI progenitors. Interestingly, we show that the E3 ubiquitin ligase, Neuralized, regulates Delta levels and Notch signalling activity at the transition zone. We demonstrate that the proneural transcription factor, Lethal of scute, is essential to induce expression of Neuralized and promote the transition from the PI progenitor to the PII progenitor state.
Our results show dynamic regulation of Notch signalling activity in the transition from neuroepithelial cells to neuroblasts. We propose a model in which Lethal of scute activates Notch signalling in a non-cell autonomous manner by regulating the expression of Neuralized, thereby promoting the progression between different neural stem cell states.
Throughout nervous system development, multipotent neural stem cells (NSCs) generate the vast diversity of neurons and glial cells present in the adult brain. In the mammalian brain, NSCs are a highly heterogeneous population that can alternate between active proliferative and quiescent states. Identifying the mechanisms that control NSC heterogeneity is essential for comprehending neurogenesis and brain regeneration.
The Drosophila optic lobe, which shares many of the features of neurogenesis in the mammalian cerebral cortex , is a simple model for understanding NSC diversity. Drosophila and vertebrate neuroepithelial (NE) cells exhibit states of amplification and differentiation [2,3,4], as well as interkinetic nuclear migration . The optic lobe develops from neuroepithelial cells that divide symmetrically, increasing their number, and then transform into neuroblasts (NBs) at a region called the ‘transition zone’ (Fig. 1B). Asymmetrically dividing neuroblasts self-renew and generate ganglion mother cells (GMCs) that divide once more to generate postmitotic neurons and/or glial cells [3, 4, 6]. The optic lobe transition zone is characterised by the progressive change of NSC states from neuroepithelial cells into neuroblasts, via two intermediate types of neuronal progenitors: PI and PII. PI progenitors express low levels of the neuroblast marker, Deadpan (Dpn), while PII progenitors are defined by the expression of the proneural gene, Lethal of scute (L’sc) [6,7,8]. In order to generate the optic lobe retinotopic map, a strict regulation of neuroepithelial cell amplification and state progression is necessary. The transition zone requires the action of several signalling pathways to regulate the expression of L’sc in a dynamic pattern described as a proneural wave [6,7,8,9] (Fig. 1a). These signalling pathways control NSC state progression, however, how they are precisely integrated is not well understood.
The Notch signalling pathway is a key regulator of cell-cell communication required for stem cell self-renewal and differentiation . When either Delta or Serrate binds to Notch on a neighbouring cell, the Notch intracellular domain (NICD) is cleaved and translocated to the nucleus, promoting the expression of target genes . Several studies indicate that Notch signalling is key for NSC maintenance in the developing and adult brain [10, 12, 13], however, Notch signalling can promote both NSC proliferation and quiescence depending on the signalling context . In the Drosophila optic lobe, Notch signalling regulates neuroepithelial cell amplification and fate maintenance in a manner similar to vertebrate NSCs. Notch signalling is activated across the entire neuroepithelium and loss of Notch function induces premature transformation of neuroepithelial cells into neuroblasts [7, 15,16,17,18,19,20,21]. Furthermore, ectopic activation of Notch signalling is sufficient to delay the transformation of neuroepithelial cells into neuroblasts [7, 19]. Although Notch function is required to maintain neuroepithelial cell fate, its signalling is essential for neuroblast proliferation [22, 23]. How this dual role of Notch signalling is regulated to allow the progressive change from neuroepithelial cells into neuroblasts is not completely understood.
Here we show that the ligand Delta (Dl) and the E3 ubiquitin ligase Neuralized (Neur) have key roles in the neuroepithelial cell to neuroblast transition. Dl and Neur are required for Notch signalling at the transition zone. We find that L’sc is sufficient to induce neur expression and the formation of ectopic transition zones. We propose a backward relay model in which L’sc controls cell autonomous as well as cell non-autonomous mechanisms to drive the neuroepithelial to neuroblast transition.
The following fly genotypes were used: E(spl)mγ-GFP , neur-lacZ/TM6B , UAS-Dl , UAS- NFL , UAS-NICD , hs-Flp; UAS-L’sc . Flip-out clones were used for misexpression and they were generated using yw, hs-Flp; tub > Stop > GAL4, UAS-nls-lacZ/Cyo, Dfd-EYFP or Act5c > Stop > GAL4, UAS-GFP; neur-lacZ/TM6B. Mutant clones were generated using hsFlp;; FRT82B, Ubi-RFP/TM6B and FRT82B, Dlrev10/TM6B  or FRT82B, neur1/TM6B .
Generation of mutant and misexpression clones
Flip-out clones and mutant clones were induced 24 h after larva hatching (ALH) and brains were dissected and stained 78 h ALH. Flip-out clones were induced for 10 min at 37 °C, whereas for mutant clone generation larvae were heat-shocked for 30 min at 37 °C. Larvae were kept at 25 °C.
Larval brains were fixed and stained as previously described . The following primary antibodies were used: rabbit anti-Ase (1:1000 from Y.N. Jan), chicken anti-β-gal (1:100 abcam), mouse anti-Dl (1:100, C594.9B Developmental Studies Hybridoma Bank, DSHB), guinea pig anti-Dpn (1:5000, from J. Skeath), chicken and rabbit anti-GFP (1:2000 abcam), rat anti-L’sc (1:5000) and anti-Notch (1:50, C17.9C6 DSHB). Alexa Fluor conjugated secondary antibodies were diluted 1:200 (Molecular Probes, Invitrogen). Primary and secondary antibodies were incubated at 4 °C overnight.
In situ hybridisation
Probes were generated by PCR amplification from a embryonic cDNA library. Reverse primers contained the T7 polymerase promoter. Neur probe were generated using the following primers: Fw 5′- ACTCGCAATCAAACCTACTAAAGC-3′ and Rv 5′- CAGTAATACGACTCACTATTA AAGTGTAATTTAAAATGCGGCTTC-3′. For tom probe we used: Fw 5′- AAATCTCAACAATCCTCAACACAA-3′ and Rv 5′- CAGTAATACGACTCACTATTA TACGAAGACCCTAACAAACAAACA-3′ .
in situ hybridisation was performed using a standard protocol. Briefly, third instar larval brains were fixed in 4% Formaldehyde in 1X PBS, washed with PBT (1X PBS, 0.1% Tween-20) and permeabilised using 50 μg/mL Proteinase K. Probes were hybridised at 55 °C, brains were blocked 30 min using 10% normal goat serum and incubated with anti-digoxigenin AP (1:2,000 Roche) for 2 h. Staining was performed using NBT/BCIP.
Images were acquired using a Leica SP5 confocal microscope or a Zeiss Axioplasm microscope with a Leica DFC420C camera. Images, diagrams and figures were assembled using Fiji, adobe Photoshop CS2 and Illustrator CS3.
E(spl)mγ reports Notch signalling in the optic lobe transition zone
Notch signalling is necessary to maintain both neuroepithelial cell and neuroblast fates. To understand the regulation of Notch signalling during the transition of neuroepithelial cells to neuroblasts, we searched for a Notch reporter that precisely reflects the activation of the pathway. Several Notch reporters have been characterised as expressed in neuroepithelial cells and neuroblasts, however, most of these express GFP or lacZ under the control of a Notch target gene promoter. Due to the stability of GFP and β-galactosidase, these reporters do not reflect rapid changes in Notch signalling. To overcome this, we used the E(spl)mγ-GFP reporter (hereinafter referred as E(spl)mγ) that contains the E(spl)mγ promoter and coding sequence fused to GFP, reflecting the dynamics of E(spl)mγ protein half-life and turnover .
E(spl)mγ was expressed at high levels at the transition zone (Fig. 1b-b”’). Interestingly, E(spl)mγ expression was completely downregulated before neuroblast formation and then reexpressed in neuroblasts (high Dpn-positive cells, see Fig. 1b’,b”’). Notch signalling downregulation correlated with high levels of Dl (Fig. 1b,b”). This expression pattern suggests that Notch signalling is highly active in PI progenitors, blocked after PII induction and restored upon neuroblast transformation (Fig. 1b).
In order to confirm that E(spl)mγ expression was regulated by Notch signalling, we generated clones misexpressing the intracellular domain of Notch (NICD), which activates Notch signalling in a cell autonomous manner. NICD clones marked with β-gal expressed high levels of E(spl)mγ, confirming that the reporter was activated by Notch signalling. Furthermore, NICD clones also expressed low levels of Dpn suggesting that PI progenitor fate is induced by Notch signalling (see arrow in Fig. 2a-a”). Therefore, E(spl)mγ expression reflects the dynamic activity of Notch signalling at the transition zone.
Notch levels control signalling activity at the transition zone
The expression of E(spl)mγ suggested a precise regulation of Notch signalling. Notch signalling was quickly blocked in one or two cells before neuroblast transformation and activated again in neuroblasts. Given that E(spl)mγ-negative cells were in direct contact with Dl-positive cells (Fig. 1b), we hypothesised that Notch signalling was regulated by the levels of receptor. We analysed the expression of Notch receptor at the transition zone (Fig. 2b,b”). Although Notch was expressed in all neuroepithelial cells, the E(spl)mγ reporter was activated only at the transition zone (Fig. 2b,b”). Interestingly, Notch and E(spl)mγ levels were reduced together at the end of the transition zone (see arrow Fig. 2b-b”) and increased after neuroblast transformation, suggesting that Notch signalling is regulated by the levels of expression of Notch.
To assess whether downregulation of Notch is the main mechanisms for blocking Notch signalling at the transition zone, we generated clones expressing a full length form of Notch (NFL). NFL clones activated the E(spl)mγ reporter only at the transition zone, while no E(spl)mγ expression was observed in clones in the middle of the neuroepithelium, where Dl is not expressed (Fig. 2c-c”). However, NFL clones that crossed the transition zone maintained expression of E(spl)mγ and low levels of Dpn, suggesting that Notch signalling was active and induced PI progenitor fate (see arrow in Fig. 2c-c”). Additionally, NFL clones that crossed the transition zone maintained L’sc expression, delaying the transformation into neuroblasts (see arrow in Fig. 2d-d”). These results suggest that Notch expression is rapidly downregulated in order to block its signalling, which is necessary to allow the precise transition from PII progenitors into neuroblasts.
Delta activates Notch signalling inducing the formation of PI progenitor state
To understand the role of Dl at the transition zone, we generated Dl misexpression clones and assessed E(spl)mγ expression. Dl misexpression blocked E(spl)mγ expression at the transition zone (Fig. 3a-a”), but activated E(spl)mγ expression and induced low levels of Dpn in neighbouring wild-type cells (see arrowheads in Fig. 3b-b”). This result suggests that Dl can activate Notch signalling, inducing PI progenitor fate in a non-cell autonomous manner, but that high levels of Dl block Notch signalling in a cell autonomous manner. However, we did not observe high levels of E(spl)mγ and Dpn surrounding the clones, suggesting that ectopic PI progenitors generated by Dl misexpression might not be competent to transform into neuroblasts.
To characterise further the role of Dl in Notch signalling, we analysed Dl mutant clones. Mutant clones for a null allele of Dl (Dlrev10)  were generated by mitotic recombination and marked by the absence of RFP expression. These clones had no detectable Dl (Fig. 3c-c") and the levels of the E(spl)mγ reporter and Dpn were decreased (see arrow in Fig. 3c-c”), suggesting that Dl is necessary for Notch signalling and PI progenitor induction at the transition zone. Interestingly, E(spl)mγ expression was also downregulated in mutant neuroblasts (see arrowhead in Fig. 3c-c’). Small Dl mutant clones were not affected and showed normal E(spl)mγ expression, suggesting that wild-type cells can rescue Notch signalling in a non-cell autonomous manner (Fig. 3d-d”’). Non-cell autonomous activation could also be observed in mutant cells of larger clones, which were adjacent to Dl expressing wild-type cells (see arrows in Fig. 3d-d”’). Together these results strongly suggest that Dl is the major ligand for Notch activation and PI progenitor state induction at the transition from neuroepithelial cells to neuroblasts.
Neuralized is required for Notch signalling at the transition zone
The E3 ubiquitin ligase neuralized (neur) [33,34,35] promotes endocytosis of the Dl ligand, activating Notch signalling in neighbouring cells [36, 37]. As Neur function has not been assessed during optic lobe development, we decided to investigate whether it participates in the regulation of Notch signalling at the transition zone. We used a lacZ insertion in the neur locus (neur-lacZ) as an expression reporter during the transition from neuroepithelial cells into neuroblasts. . neur-lacZ expression was observed at the end of the transition zone and in optic lobe neuroblasts. Neur is initiated in the second of the L’sc expressing PII progenitors, just prior to their transformation into Dpn positive neuroblasts (Fig. 4a). These medial PII progenitors also expressed Dl (see arrowhead in Fig. 4a-a”’), but at lower levels than the most lateral PII progenitor. We observed high levels of neur mRNA at the transition zone, in a pattern complementary to twin of m4 (tom) expression, a Notch target gene expressed across the neuroepithelium  (Additional file 1: Figure S1). Therefore, neur is expressed in medial PII progenitors and in optic lobe neuroblasts (Fig. 4b).
To assess Neur function, we generated neur mutant clones using a null allele (neur1) . Mutant clones showed a reduction in E(spl)mγ expression in a cell autonomous manner (Fig. 5a-a”’) resembling Dl mutant clones (compare to Fig. 3a-a”’). The reduction in E(spl)mγ expression was observed in optic lobe neuroblasts (69.7% of clones, 23/33, see arrowhead in Fig. 5a-a”) and also in PI progenitors (52.0% of clones, 13/25), in which Dpn levels were also reduced (see arrow in Fig. 5a”). In addition, while L’sc levels were normal in neur mutant cells, Dl levels were upregulated in clones at the transition zone and in Dpn-positive neuroblasts (Fig. 5a”’ and 5b”). This suggests that neur is required to activate Dl-mediated Notch signalling and to induce PI progenitor state in the neuroepithelial to neuroblasts transition zone.
Lethal of scute is sufficient to induce neuralized expression and to generate ectopic transition zones
neur was expressed preferentially in the L’sc-positive PII progenitors closest to neuroblasts (Fig. 5). PII progenitor fate is defined by the expression of L’sc , hence we hypothesised that L’sc regulates neur expression in order to activate Notch signalling and induce PI progenitor fate. To test this, we generated L’sc misexpression clones outside the transition zone. L’sc misexpression was sufficient to induce neur expression in neuroepithelial cells (see arrows in Fig. 6a-a”’). Remarkably, L‘sc misexpression generated ectopic transition zones in the neuroepithelium (Fig. 6b). These clones showed high levels of Dpn and Neur, and a decrease in Notch receptor levels, demonstrating that ectopic neuroblasts were generated by L’sc misexpression (Fig. 6b-b”’).
Interestingly, neur expression was also observed outside the clones (see arrow in Fig. 6b-b”) as were PI progenitors (low Dpn-positive cells; Fig. 6b”). In some cases, L’sc misexpressing cells were found deep inside the optic lobe (see blue clone in Additional file 2: Figure S2), suggesting that the L’sc misexpressing cells initiated the ectopic transition zone and then delaminated from the neuroepithelium after neuroblast transformation. The ectopic transition zones remained in the neuroepithelium after the clones had delaminated (note lack of GFP expression in Fig. 6c). These ectopic transition zones contained Dpn-positive PI progenitors, Neur-positive PII progenitors and Dpn-positive/Neur-positive neuroblasts.
We conclude that the induction of L’sc within the neuroepithelium is sufficient to induce neur expression and to generate ectopic transition zones containing PI and PII progenitor states in a non-cell autonomous manner. Remarkably these ectopic transition zones are maintained and continue to generate neuroblasts.
Notch signalling acitivity is dynamically regulated in the transition zone. The E(spl)mγ reporter is highly expressed in PI progenitor cells, downregulated in PII progenitor cells and upregulated again in neuroblasts [8, 38]. Here, we demonstrate that the ligand Delta and the E3 Ubiquitin ligase Neur are required in PII progenitor cells to activate Notch signalling in neighbouring PI progenitors. We also show that Neur expression is induced by the proneural factor L’sc, which is able to induce the entire transition zone.
A switch from serrate to Delta mediates Notch signalling in the progression of neural stem cell states
Notch mutant clones are extruded from the neuroepithelium and prematurely transform into neuroblasts at ectopic positions . Interestingly, Dl mutant clones in the lateral neuroepithelium do not phenocopy these Notch null mutant clones . This suggests that Dl is not required for Notch signalling in more lateral proliferating neuroepithelial cells and that Notch is activated by a different ligand. Indeed, Perez-Gomez et al.  showed that glial cells adjacent to the neuroepithelium activate Notch signalling via the ligand Serrate (Ser). Ser is necessary for neuroepithelial cell proliferation and for preventing PII progenitor formation . Hence, we favour a model in which Notch signalling induce by Serrate maintains neuroepithelial cells in a proliferating state, while Notch signalling induced by Delta initiates PI progenitor formation and the neuroepithelial cell to neuroblast transition.
The differential expression of Notch signalling modulators, such as the protein Canoe (Cno), may explain preferential binding for one of the two ligands. Canoe stabilises the Notch receptor at adherens junctions and promotes binding to Ser from glial cells . The E3 ubiquitin ligase, Mind bomb, is required for the activation of Ser while Neur controls the activity of Delta .
We show that neur expression is restricted to PII progenitors cells closest to the neuroblasts (Fig. 4). However, the loss of neur affects cells that are not immediate neighbours, the PI progenitors, implying that Delta-Notch signalling may work over a distance. Membrane protrusions may allow Dl to activate N signalling at a distance, as has been described during bristle development [40, 41] (Fig. 7a). Alternatively, it has been shown that Notch signalling promotes Dl expression . This positive feedback loop may allow the initial Neur activity to propagate in a non-cell autonomous manner, generating a gradient of Notch signalling (Fig. 7b).
A backward relay mechanism controls changes in neural stem cell states
Neuroepithelial cells progress through two transient progenitor states prior to transforming into medulla neuroblasts [6,7,8]. Here we show that PII progenitors can be further subdivided into L’sc, high Delta and L’sc, Neur expressing cells (Fig. 7). Two opposing signalling pathways control the medial to lateral progression of the proneural wave that initiates the neuroepithelial cell to neuroblast transition. EGF signalling drives the wave forward, while JAK/STAT signalling slows the progression of the wave [7, 9, 42] and prevents ectopic neuroblast formation in the epithelium . PII progenitor cells secrete the EGF ligand, Spitz, which activates the EGFR pathway in neighbouring lateral PI progenitor cells. These neuroepithelial cells are positive for the EGFR downstream target gene pointed P1 (pntP1). pnt or spitz loss-of-function mutant clones do not upregulate L’sc, indicating that both the induction of the proneural wave, and its progression, are downstream of EGFR signalling . Moreover, the EGF signalling controls the levels of Dl ligand, regulating Notch signalling and the progression of the proneural wave [7, 44].
L’sc acts in a backward relay mechanism to induce the PI progenitor state. It induces the expression of Neur in PII progenitors and thus activates Delta-Notch signalling to induce PI. As a result neighbouring PI progenitors upregulate the Notch target gene E(spl)mγ. One role of high Notch signalling activity in PI is to induce cell cycle arrest in PI progenitor cells . Hence, the backward relay mechanism controls the sequential and timely acquisition of progenitor states.
In order for neuroepithelial cells to transform into neuroblasts, Notch signalling must be blocked. Binding of Dl to Notch in the same cell can inhibit Notch signalling through a mechanism called ‘cis-inhibition’ . We observed high Dl levels in PII progenitor cells where E(spl)mγ levels are low. Furthermore, we show that Dl misexpression clones show no Notch signalling activity. Therefore, it is plausible that Dl activates Notch in trans, inducing the PI progenitor state, while inhibiting Notch in cis to enable the progression from PII progenitors to neuroblasts.
Notch signalling regulates stem cell heterogeneity from flies to vertebrates
The Notch signalling pathway regulates stem cell maintenance, proliferation and differentiation in different tissues, contributing to vertebrate development and organ regeneration. However, the effect of Notch signalling is highly dependent on the biological context . During development and adult neurogenesis, NSCs are a highly heterogeneous population. NSCs can be found in proliferative or quiescent states. Furthermore, adult NSCs generate intermediate progenitor states with different potency before differentiation into neurons or glial cells . Notch signalling preserves NSC maintenance and proliferation [47,48,49] and can also induce the quiescence state [14, 50,51,52]. The context of Notch signalling in NSCs determines the outcome. For example in zebrafish, whereas the Notch3 receptor induces a quiescence state in NSCs, Notch1b is required for NSC population maintenance . This phenomenon resembles the different responses to Notch signalling in neuroepithelial cells in the Drosophila optic lobe.
Notch signalling interaction with other pathways also regulates NSC behaviour in the vertebrate brain. EGFR signalling in neural progenitors non-autonomously blocks Notch signalling in NSCs, reducing NSC proliferation at the adult subventricular zone . Interestingly, EGFR is a downstream target of Notch signalling in NSCs , suggesting that Notch promotes both NSC maintenance and the formation of neural progenitors.
Our study proposes a model of dynamic Notch signalling in the transition from neuroepithelial cells into neuroblasts. During Drosophila optic lobe development, Notch signalling regulates NSC amplification and maintenance in a similar manner to vertebrate NSCs. Notch signalling also induces the progression into PI/PII progenitor states. Understanding the dynamic regulation of Notch signalling during NSC state transitions in the optic lobe may yield new insights into the mechanisms that control adult neurogenesis and brain regeneration.
We would like to thank Sarah Bray, François Schweisguth, Eugenia Piddini, Pat Simpson, Yuh Nung Jan, Jim Skeath and DSHB for antibodies and fly stocks. We thank Takumi Suzuki and Carlos Oliva for comments on the manusctript.
Lethal of scute
Notch intracellular domain
Neural stem cell
Brand AH, Livesey FJ. Neural stem cell biology in vertebrates and invertebrates: more alike than different? Neuron. 2011;70(4):719–29.
Lanet E, Gould A, Maurange C. Protection of neuronal diversity at the expense of neuronal numbers during nutrient restriction in the Drosophila visual system. Cell Rep. 2013;3(3):587–94.
Egger B, Boone JQ, Stevens NR, Brand AH, Doe CQ. Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe. Neural Dev. 2007;2:1.
Hofbauer A, Campos-Ortega J. Proliferation pattern and early differentiation of the optic lobes in Drosophila melanogaster. Roux Arch Dev Biol. 1990;198(5):264–74.
Rujano MA, Sanchez-Pulido L, Pennetier C, le Dez G, Basto R. The microcephaly protein Asp regulates neuroepithelium morphogenesis by controlling the spatial distribution of myosin II. Nat Cell Biol. 2013;15(11):1294–306.
Sato M, Suzuki T, Nakai Y. Waves of differentiation in the fly visual system. Dev Biol. 2013;380(1):1–11.
Yasugi T, Sugie A, Umetsu D, Tabata T. Coordinated sequential action of EGFR and Notch signaling pathways regulates proneural wave progression in the Drosophila optic lobe. Development. 2010;137(19):1–11.
Egger B, Gold KS, Brand AH. Regulating the balance between symmetric and asymmetric stem cell division in the developing brain. Fly. 2011;5(3):237–41.
Yasugi T, Umetsu D, Murakami S, Sato M, Tabata T. Drosophila optic lobe neuroblasts triggered by a wave of proneural gene expression that is negatively regulated by JAK/STAT. Development. 2008;135(8):1471–80.
Koch U, Lehal R, Radtke F. Stem cells living with a Notch. Development. 2013;140(4):689–704.
Bray SJ. Notch signalling: a simple pathway becomes complex. Nat Rev Mol Cell Biol. 2006;7(9):678–89.
Pierfelice T, Alberi L, Gaiano N. Notch in the vertebrate nervous system: an old dog with new tricks. Neuron. 2011;69(5):840–55.
Ables JL, Breunig JJ, Eisch AJ, Rakic P. Not(ch) just development: Notch signalling in the adult brain. Nat Rev Neurosci. 2011;12(5):269–83.
Giachino C, Taylor V. Notching up neural stem cell homogeneity in homeostasis and disease. Front Neurosci. 2014;8:32.
Perez-Gomez R, Slovakova J, Rives-Quinto N, Krejci A, Carmena A. A Serrate-Notch-Canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. J Cell Sci. 2013;126(Pt 21):4873–84.
Egger B, Gold KS, Brand AH. Notch regulates the switch from symmetric to asymmetric neural stem cell division in the Drosophila optic lobe. Development. 2010;137(18):2981–7.
Ngo KT, Wang J, Junker M, Kriz S, Vo G, Asem B, Olson JM, Banerjee U, Hartenstein V. Concomitant requirement for Notch and Jak/Stat signaling during neuro-epithelial differentiation in the Drosophila optic lobe. Dev Biol. 2010;346(2):284–95.
Orihara-Ono M, Toriya M, Nakao K, Okano H. Downregulation of Notch mediates the seamless transition of individual Drosophila neuroepithelial progenitors into optic medullar neuroblasts during prolonged G1. Dev Biol. 2011;351(1):163–75.
Wang W, Liu W, Wang Y, Zhou L, Tang X, Luo H. Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development. Dev Biol. 2011;350(2):414–28.
Reddy BV, Rauskolb C, Irvine KD. Influence of fat-hippo and notch signaling on the proliferation and differentiation of Drosophila optic neuroepithelia. Development. 2010;137(14):2397–408.
Green P, Hartenstein AY, Hartenstein V. The embryonic development of the Drosophila visual system. Cell Tissue Res. 1993;273(3):583–98.
Zacharioudaki E, Magadi SS, Delidakis C. bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation. Development. 2012;139(7):1258–69.
Zhu S, Wildonger J, Barshow S, Younger S, Huang Y, Lee T. The bHLH repressor deadpan regulates the self-renewal and specification of Drosophila larval neural stem cells independently of Notch. PLoS One. 2012;7(10):e46724–15.
Almeida MS, Bray SJ. Regulation of post-embryonic neuroblasts by Drosophila Grainyhead. Mech Dev. 2005;122(12):1282–93.
Phillips RG, Roberts IJ, Ingham PW, Whittle JR. The Drosophila segment polarity gene patched is involved in a position-signalling mechanism in imaginal discs. Development. 1990;110(1):105–14.
Doherty D, Feger G, Younger-Shepherd S, Jan LY, Jan YN. Delta is a ventral to dorsal signal complementary to serrate, another Notch ligand, in Drosophila wing formation. Genes Dev. 1996;10(4):421–34.
Zecchini V, Brennan K, Martinez-Arias A. An activity of Notch regulates JNK signalling and affects dorsal closure in Drosophila. Curr Biol. 1999;9(9):460–9.
Cooper MT, Bray SJ. R7 photoreceptor specification requires Notch activity. Curr Biol. 2000;10(23):1507–10.
Carmena A, Bate M, Jimenez F. Lethal of Scute, a proneural gene, participates in the specification of muscle progenitors during Drosophila embryogenesis. Genes Dev. 1995;9(19):2373–83.
Haenlin M, Kramatschek B, Campos-Ortega JA. The pattern of transcription of the neurogenic gene Delta of Drosophila melanogaster. Development. 1990;110(3):905–14.
Lehmann R, Jiménez F, Dietrich U, Campos-Ortega J. On the phenotype and development of mutants of early neurogenesis inDrosophila melanogaster. Wilehm Roux Arch Dev Biol. 1983;192(2):62–74.
Van Vactor DL Jr, Cagan RL, Kramer H, Zipursky SL. Induction in the developing compound eye of Drosophila: multiple mechanisms restrict R7 induction to a single retinal precursor cell. Cell. 1991;67(6):1145–55.
Yeh E, Dermer M, Commisso C, Zhou L, McGlade CJ, Boulianne GL. Neuralized functions as an E3 ubiquitin ligase during Drosophila development. Curr Biol. 2001;11(21):1675–9.
Lai EC, Deblandre GA, Kintner C, Rubin GM. Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta. Dev Cell. 2001;1(6):783–94.
Deblandre GA, Lai EC, Kintner C. Xenopus neuralized is a ubiquitin ligase that interacts with XDelta1 and regulates Notch signaling. Dev Cell. 2001;1(6):795–806.
Le Borgne R, Schweisguth F. Unequal segregation of Neuralized biases Notch activation during asymmetric cell division. Dev Cell. 2003;5(1):139–48.
Pavlopoulos E, Pitsouli C, Klueg KM, Muskavitch MA, Moschonas NK, Delidakis C. neuralized encodes a peripheral membrane protein involved in delta signaling and endocytosis. Dev Cell. 2001;1(6):807–16.
Weng M, Haenfler J, Lee C. Changes in Notch signaling coordinates maintenance and differentiation of the Drosophila larval optic lobe neuroepithelia. Dev Neurobiol. 2012;72(11):1376–90.
Le Borgne R, Remaud S, Hamel S, Schweisguth F. Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila. PLoS Biol. 2005;3(4):e96.
De Joussineau C, Soule J, Martin M, Anguille C, Montcourrier P, Alexandre D. Delta-promoted filopodia mediate long-range lateral inhibition in Drosophila. Nature. 2003;426(6966):555–9.
Cohen M, Georgiou M, Stevenson NL, Miodownik M, Baum B. Dynamic filopodia transmit intermittent Delta-Notch signaling to drive pattern refinement during lateral inhibition. Dev Cell. 2010;19(1):78–89.
Wang W, Li Y, Zhou L, Yue H, Luo H. Role of JAK/STAT signaling in neuroepithelial stem cell maintenance and proliferation in the Drosophila optic lobe. Biochem Biophys Res Commun. 2011;410(4):714–20.
Tanaka Y, Yasugi T, Nagayama M, Sato M, Ei SI. JAK/STAT guarantees robust neural stem cell differentiation by shutting off biological noise. Sci Rep. 2018;8(1):12484.
Sato M, Yasugi T, Minami Y, Miura T, Nagayama M. Notch-mediated lateral inhibition regulates proneural wave propagation when combined with EGF-mediated reaction diffusion. Proc Natl Acad Sci U S A. 2016;113(35):E5153–62.
del Alamo D, Rouault H, Schweisguth F. Mechanism and significance of cis-inhibition in Notch signalling. Curr Biol. 2011;21(1):R40–7.
Farkas LM, Farkas LM, Huttner WB, Huttner WB. The cell biology of neural stem and progenitor cells and its significance for their proliferation versus differentiation during mammalian brain development. Curr Opin Cell Biol. 2008;20(6):707–15.
Imayoshi I, Sakamoto M, Yamaguchi M, Mori K, Kageyama R. Essential roles of Notch signaling in maintenance of neural stem cells in developing and adult brains. J Neurosci. 2010;30(9):3489–98.
Andreu-Agullo C, Morante-Redolat JM, Delgado AC, Farinas I. Vascular niche factor PEDF modulates Notch-dependent stemness in the adult subependymal zone. Nat Neurosci. 2009;12(12):1514–23.
Ehm O, Goritz C, Covic M, Schaffner I, Schwarz TJ, Karaca E, Kempkes B, Kremmer E, Pfrieger FW, Espinosa L, et al. RBPJkappa-dependent signaling is essential for long-term maintenance of neural stem cells in the adult hippocampus. J Neurosci. 2010;30(41):13794–807.
Carlen M, Meletis K, Goritz C, Darsalia V, Evergren E, Tanigaki K, Amendola M, Barnabe-Heider F, Yeung MS, Naldini L, et al. Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. Nat Neurosci. 2009;12(3):259–67.
Chapouton P, Skupien P, Hesl B, Coolen M, Moore JC, Madelaine R, Kremmer E, Faus-Kessler T, Blader P, Lawson ND, et al. Notch activity levels control the balance between quiescence and recruitment of adult neural stem cells. J Neurosci. 2010;30(23):7961–74.
Katz S, Cussigh D, Urban N, Blomfield I, Guillemot F, Bally-Cuif L, Coolen M. A nuclear role for miR-9 and Argonaute proteins in balancing quiescent and activated neural stem cell states. Cell Rep. 2016;17(5):1383–98.
Alunni A, Krecsmarik M, Bosco A, Galant S, Pan L, Moens CB, Bally-Cuif L. Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium. Development. 2013;140(16):3335–47.
Aguirre A, Rubio ME, Gallo V. Notch and EGFR pathway interaction regulates neural stem cell number and self-renewal. Nature. 2010;467(7313):323–7.
This work was funded by the Royal Society Darwin Trust Research Professorship, Wellcome Trust Senior Investigator Award 103792 and Wellcome Trust Programme grant 092545 to A.H.B., Wellcome Trust 4-year PhD Studentships to E.G.C. and K.S.G. and a Fellowship from the Swiss National Foundation to B.E. A.H.B acknowledges core funding to the Gurdon Institute from the Wellcome Trust (092096) and CRUK (C6946/A14492).
Availability of data and materials
All data is available upon request.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Figure S1. neur is expressed in the transition zone. in situ hybridisation of larval brains using specific antisense probes against (A) tom and (B) neur. Note that neur and tom are expressed in a complementary pattern. (PSD 81600 kb)
Figure S2. L’sc misexpression clones delaminate and induce ectopic transition zones in wild-type neuroepithelial cells. (A-A”) L’sc misexpressing clone that has delaminated from the neuroepithelium induces an ectopic transition zone. Staining for (A,A’) β-gal/neur in green, (A,A”) Dpn in red and (A) Notch receptor in gray. Clone is marked by GFP expression in blue. Note that the clone has delaminated inside the optic lobe and the ectopic transition zone has continued generating NBs. (PSD 12135 kb)