Fig. 7
From: Different Fgfs have distinct roles in regulating neurogenesis after spinal cord injury in zebrafish
Fgfs increase neural proliferation and neurite outgrowth in mammalian PC12 cells. a Kinetics of MAPK activation (p-MAPK) shown in Western blots in control (c) or 1, 2 or 4 h following treatment with Fgf2, Fgf3 and Fgf8. Rapid activation of MAPK signaling occurs in response to all three Fgfs, but this activation is only transient in response to Fgf3 treatment as opposed to Fgf2 and Fgf8 treatment, which drives longer term activation. Total amount of MAPK is indicated by blotting the membrane with MAPK antibody. b Representative images of control versus Fgf2, 3 and 8 treated PC12 cells showing Ki67 immunostaining (green) labelling proliferation and b-tubulin immunostaining (red) labelling neurite morphology. c Quantitation of Ki67+ proliferative cells as a percentage of total DAPI nuclear cell counts shows significant increases in proliferation following treatment with any of the Fgfs. Results are presented as mean ± SEM, *** p < 0.001. d Neurite length sorted from shortest to longest show increased neurite length in cells treated with Fgf2 and Fgf8 but not Fgf3 compared to control. Neurite length *** p < 0.001