Fig. 2
Activity of GFP reporters relative to photoreceptors in the chicken retina. a Schematic of the Stagia3 reporter vector Arrow represents transcription initiation position and direction. b Schematic of the Nucβ-gal driven by the CAG promoter element co-electroporation control. c E5 Retinas electroporated with a CAG::Nucβ-gal and the GFP reporter shown to the left of panels, explanted and cultured for 2 days, and imaged by confocal microscopy for the expression of GFP (green, rabbit antibody), Nuc β-gal (orange, chicken antibody), and Visinin (purple). The scleral portion of the retina is located near the top of the image. Scale bar in top left panel represents 20 μm and applies to all panels. Abbreviations: ECR (Evolutionarily Conserved Region) TATA (TATA box), EGFP (Enhanced Green Fluorescent Protein), ires (Internal Ribosomal Entry Site), PLAP (Placental Alkaline Phosphatase), Nucβ-gal (Nuclear β-galactosidase)