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Fig. 9 | Neural Development

Fig. 9

From: DSCAM differentially modulates pre- and postsynaptic structural and functional central connectivity during visual system wiring

Fig. 9

DSCAM downregulation differentially influences RGC and bipolar cell dendrite growth. Dendritic morphologies of fluorescently labeled (a-c) RGCs and (d-g) bipolar cells (BCs) transfected with Control MO or DSCAM MO are illustrated by the confocal projections of stage 45 retina cryostat sections (a, d, e) and sample three-dimensional tracings (b, f). Sections in (a and e) were counterstained with DAPI to reveal the retinal layers. Inl, inner nuclear layer; gcl, ganglion cell layer; onl, outer nuclear layer. c The number of dendritic branches, total dendritic arbor length, number of dendritic crossings, and number of fasciculated dendritic bundles of RGCs treated with DSCAM MO (n = 18) were compared to those treated with Control MO (n = 13). No significant differences were found across each category. g Morphological analysis of neurons traced three-dimensionally reveals a significant increase in the number of dendritic branches and total dendritic arbor length of bipolar cells in response to DSCAM downregulation. BCs treated with DSCAM MO also showed a significant increase in the number of dendritic crossings and fasciculated bundles compared to cells treated with control MO. DSCAM MO (n = 28), Control MO (n = 42). Error bars indicate mean ± SEM. ***p < 0.001, or ns for no significance. Scale bars: 10 μm in (a, b); 20 μm in (d-f)

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