Fig. 8

DSCAM downregulation decreases RGC axon arbor growth cell autonomously. a Sample axon arbors from RGCs transfected with Control MO or DSCAM MO together with Alexa 488 dextran and imaged at stage 45, and 24 and 48 h after initial imaging. b-d Quantitative analysis of axon branch number (b) and total axon arbor length (c) demonstrate that in contrast Control MO transfected RGCs, axons from RGCs with DSCAM knockdown failed to increase their number of branches over time, an effect that significantly decreased axon arbor growth rate (d; change in branch number and length). e Sholl analysis revealed no significant differences in the branching patterns of RGC axons with DSCAM knockdown each imaging time point when compared to controls. Control MO (n = 15) or DSCAM MO (n = 18). Comparisons are by Two-way ANOVA and Student’s-t-test. Error bars indicate mean ± SEM. * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.001. Scale bars: 20 μm in (a)