Fig. 6

Downregulation of DSCAM expression in the optic tectum affects visually guided behavior. a Fluorescein-tagged Control MO or DSCAM MO was bulk electroporated into the caudal midbrain region of stage 43 tadpoles. Fluorescence microscopy imaging was used to confirm bilateral MO transfection into the optic tectum at stage 45. b Schematic of the visual avoidance task. The tadpole’s response to the advancing stimuli (black to gray circle) results in the tadpole changing its swimming direction (red arrows). c Tadpoles electroporated with DSCAM MO had decreased avoidance responses to the presentation of the stimulus 24 h post-treatment when compared to uninjected controls, vehicle injected controls, and Control MO electroporated tadpoles (Student’s t-test). Error bars indicate mean ± SEM. * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.001. Scale bars: 100 μm (a)