Fig. 3
From: Identification and characterization of mushroom body neurons that regulate fat storage in Drosophila
Behavioral and metabolic phenotypes associated with silenced and hyperactivation of the different mushroom body circuits involved in fat storage regulation. a Food intake in flies with silenced or hyperactivated MB. Silencing of any type of mushroom body-associated neuron does not significantly affect food intake (upper row). Hyperactivation of α’β’ KCs, using VT30604-GAL4, (lower left) of MBON-γ5β2’a, MBON-β2’mp (MB011B-GAL4 neurons; lower right) cause increases in food intake; all other genotypes are not significantly different from controls. b Conversion of ingested 14C aspartic acid to protein (magenta), carbohydrate (light blue), and lipid (yellow) in flies with silenced or hyperactivated MB neurons. Silencing of α’β’ KCs (VT30604), MBON-γ2α’1 (MB077B), MBON-α’2 (MB082C), and PAM-γ5 (MB315C) produces decreases in labeled lipids, and silencing γ KCs (MB009B) produces an increase (upper panels). Hyperactivating γ KCs, using MB009-GAL4, and DPM neurons, using C316-GAL4, produces decreases in labeled lipids (lower panels). Bars indicate means ± SEM, n = 60 single flies in pooled controls (see Fig. 2) and n = 20 for all other genotypes for Café and climbing assay, and n = 12 for pooled controls and n = 4 for other genotypes for the fat store degradation and 14C incorporation experiments (each sample is a homogenate from 10 flies). Asterisks denote t-test statistical significance: *p < 0.05, **p < 0.005, ***, p < 0.0005