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Fig. 2 | Neural Development

Fig. 2

From: Identification and characterization of mushroom body neurons that regulate fat storage in Drosophila

Fig. 2

Mushroom body input and output neurons involved in fat storage regulation. a Input neurons. Quantitation of fat levels, as measured by TLC, in flies with Kir2.1-mediated silencing (left) or dTrpA1-mediated hyperactivation (right) of DPM neurons using C316-GAL4, or of PAM-γ5 neurons using MB315-GAL4. Far right, Image of PAM-γ5 neurons (orange). In all images in this figure, the brain is a translucent grey skeleton, and the MB lobes are in translucent pink and blue. There are 8–12 PAM-γ5s per brain hemisphere. b MBONs innervating α’ compartments. Left bar graph shows that Kir2.1-mediated silencing of MBON-γ2α’1 using MB077B-GAL4 and MBON-α’2 using MB082C-GAL4 causes leanness. Right bar graph shows that dTrpA1-mediated hyperactivation of these neurons has no effect. Far right, images of MBON-γ2α’1 (blue-green) and MBON-α’2 (light green). There are 2 MBON-γ2α’1 s and 1 MBON-α’2, per brain hemisphere. c MBONs innervating β’2 compartments. Left bar graph shows that Kir2.1-mediated silencing of MBON-γ5β’2a and MBON-β’2mp neurons using MB011B-GAL4 has no effect on fat content. Right bar graph shows that dTrpA1-mediated hyperactivation of the same neurons causes obesity. Far right, images of MBON-γ5β’2a (red) and MBON-β’2mp (blue). There is 1 MBON-γ5β’2a and 1 MBON-β’2mp per brain hemisphere. d Combined images at the bottom show superimpositions of all α’-innervating MBONs (left), all β’-innervating MBONs (middle), all MBONs (right), and MBONs plus PAM-γ5s (bottom). Bars indicate means ± SEM, n = 12 samples for pooled controls as in Fig. 2 and n = 4 for other genotypes, each composed of 10 flies homogenate. Asterisks denote t-test statistical significance: ***, p < 0.0005

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