Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

Table 2 Summary of transmission and viability of mutant lines

Gene name	F0 generation	F1 generation	F2 generation
gas6	5 fish screened, 2 founders	Founder 1: 12/60 carried mutations; transmitted 4 different alleles, each resulting in a frame-shift mutation Founder 2: 0/92 carried mutations	5/16 homozygous mutant
gbx1	2 fish screened, 2 founders,	Founder 1: 34/48 carried mutations; transmitted 4 different alleles – 2 resulted in frame-shift mutations, 2 did not Founder 2: did not produce offspring	0/15 homozygous mutant
sall4	N/A	^a8/24	5/21 homozygous mutant
greb1l	N/A	^a10/24	5/34 homozygous mutant
celf2	N/A	^a12/24	^b1/21 homozygous mutant
egfl6	N/A	^a16/24	^c6/24 homozygous mutant

^aZIRC provided offspring of a F1 heterozygous carrier and a WT fish; thus, 50% should be heterozygous carriers
^b1 homozygous fish identified, it was crossed with a heterozygous sibling for all in situ analyses
^chomozygous mutants do not breed, all in situ analyses were thus done on crosses of heterozygous carriers

ISSN: 1749-8104