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Fig. 4 | Neural Development

Fig. 4

From: Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

Fig. 4

Expression of the r5/r6 gene-set requires FGF and RA signaling. Expression of r5/r6 genes was assayed via ISH in (i) WT embryos treated with 50uM DMSO, (ii) WT embryos treated with 50uM SU5402 and (iii) WT embryos treated with 10uM DEAB. The genes assayed include a tox3, b sema3fb, c mpz, d gas6, e celf2, f nr2f2, g col15a1b and h gbx1. krox20 (red), which is expressed in r3 and r5, was used to position the expression domain of some genes as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging

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