Fig. 4From: Tcf7L2 is essential for neurogenesis in the developing mouse neocortexThe loss of Tcf7L2 in the embryonic cortex leads to downregualtion of canonical Wnt signalling and reduction of radial glial cells and intermediate progenitors. a-d‘ β–galactosidase immunofluorescence in the cortical VZ in BAT-Gal Wnt reporter mice is reduced after inactivation of Tcf7L2 alone (c-c‘) or upon simultaneous deletion of Tcf7L1 and Tcf7L2 (d-d‘). Inactivation of Tcf7L1 alone had a little effect on BAT-Gal Wnt reporter (b-b“). Panels in a‘ display magnified framed rectangles in a. e-g Quantifications of BAT-Gal+, Sox2+ RGCs and Tbr2+ IPCs showing an average from three independent experiments with standard deviations. Student’s paired t-test: *p < 0.05, **p < 0.01, ***p < 0.001 compared to controls, n = 6. P-values between Tcf7L2 and Tcf7L1/Tcf7L2 mutants are: BAT-Gal+ p = 0.06, Sox2+ p = 0.34, Tbr2+ p = 0.80 showing no statistical significance. h-k“ Double immunofluorescent labelling of Sox2+ RGC and Tbr2+ IPCs. Dashed lines represent potential ventricular liningBack to article page