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Fig. 2 | Neural Development

Fig. 2

From: Transcriptional regulation of ependymal cell maturation within the postnatal brain

Fig. 2

Ependymal cell maturation is delayed in the absence of Nfix. a-p Coronal sections of P0 (a, b), P5 (c-f) and P15 (g-p) mouse brains immunolabelled with antibodies against FOXJ1 (a-d, i-l) and s100β (e, f, m-p). The dotted lines indicate the ventricular wall. At P0 in the wild-type, FOXJ1 cells were seen lining the ventricles (arrows in a). In the mutant at this age, however, there were fewer of these cells (arrowheads in b). Similarly, at P5 there were fewer cells expressing either FOXJ1 (arrowheads in d) or s100β (arrowheads in f) in comparison to the controls (arrows in c, e). (g, h) Low magnification images of wild-type (g) and Nfix−/− (h) P15 cerebral cortices labelled with DAPI, showing the areas from which the higher magnification images were taken. The lateral ventricle (LV) was significantly expanded in the mutant. In the wild-type, the ependymal cell layer was clearly labelled with either FOXJ1 or s100β (arrows in i and m respectively), revealing a uniform epithelial sheet. In the mutant, however, we observed regions in which there were reduced numbers of ependymal cells lining the ventricle (arrowheads in j and n), areas where the ependymal cell layer had sloughed off (double arrowheads in k and o), and areas in which the ependymal cell layer was more than one-cell thick (l and p). q, r Cell counts demonstrated that there were significantly fewer FOXJ1-expressing (q) and s100β-expressing (r) cells per unit length at P5 in the mutant, and, conversely, that there were significantly more of these cells in the mutant at P15 in comparison to the controls. * p < 0.05, t-test. Scale bar (in g): g, h 500 μm; a-f, i-p 30 μm

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