Fig. 3

Spatial patterning of cones precedes planar polarized Crumbs localization. (A-A”) Retinal margin in a flat-mount preparation, immunolabeled for Crb2b (magenta) and ZO1 (green). (A) Single z-level focal plane of the Crb2b channel at the level of ZO1 staining (OLM) in the pre-column zone. Crb2b ‘ladders’ (arrow) in the pre-column zone and adjacent columns. (Note that due to curvature of the retinal surface, the equivalent ZO1 level in the central retina at the left is at a deeper focal plane.) Immature UV cone profiles (white dots) in the pre-column zone. (A’) The maximum intensity z-projection of ZO1 labeling. (A”) Merged image. (B-B”) Retinal margin in a flat-mount preparation, immunolabeled for Crb2a (magenta) with the trβ2: tdTomato (green) Red cone marker. (B) Note small rings of strong Crb2a staining between cone columns (inset, arrows). (B’, B”) The intensely-labeled Crb2a+ rings are not co-labeled with trβ2: tdTomato. (C-C”) Immunoreactivity for Crb2a (magenta) with the rh1: EGFP (green) rod marker. (C’, C”) The strongly immunoreactive Crb2a+ rings surround rh1: EGFP+ labeled rod photoreceptors. Scale bars: 10 μm (A”, B”, C”)